電話：34253
信箱：higuchi@ncu.edu.tw
研究室：工四館 E3-329
實驗室：生醫工程實驗室 E3-320A
學歷：日本東京工業大學工學博士
研究領域：材料與膜於生物醫學應用、幹細胞分離純化於再生、醫學應用、生物材料檢定、細胞工學

 研究背景
Prof. Higuchi is also jointed to a guest researcher in RIKEN Advanced Science Institute, National Research Institute for Child Health and Development, and a visiting professor in King Saud University. He received his Ph.D. in Tokyo Institute of Technology in 1985. He was a Professor in Seikei University from 1993 to 2007. He received Sofue Memorial Award from Society of Fiber Science, Japan in 1994, Seikei Academic Award from Seikei Alumni Association in 2003, and Nanotechnology Outsatnding Contribution Award (奈米科技傑出貢獻獎), National Science Council, Taiwan in 2013. He is interested in the development of materials for stem cell research. He established purification method of hematopoietic stem cells and mesenchymal stem cells from umbilical cord blood and adipose tissue, respectively by filtration method through polymeric porous membranes. He is also developing biomaterials for stem cell culture. He published three articles in Chemical Reviews as first and corresponding author, which has high impact factor of 40.2 higher than Nature or Science and two articles in Progress in Polymer Science (impact factor=26.4).

Professional Activity of Scientific Society:

2011-present    Editorial Board Member of World Journal of Stem Cells (WJSC, China)

2011-present   Councillar of Chemical Society of Japan, Division of Polymer Science

2012-present    Editorial Board Member of Journal of Membrane Separation Technology (Lifescience Global, Canada) 

2014-present  Associate Editor ofPhysics and Chemistry of Stem Cells (Walter de Gruyter, German)

2013-present  Editor in Chief ofAmerican Journal of Tissue Engineering & Stem Cell (Columbia International Publishing, USA)

2014-present   Advisory Board of Journal of Materials Chemistry B (Royal Society of Chemistry, SCI journal, impact factor=6.101)

2014-present   The Asia-Pacific (AP) Council of the Tissue Engineering and Regenerative Medicine International Society (TERMIS) 

2014-present   Editorial Board Member ofJournal of Stem Cells Research, Development and Therapy (Herald Scholarly Open Access, USA)

2014-present   Editorial Board Member ofJournal of Cytology and Tissue Biology (Herald Scholarly Open Access, USA)

 論文著作

Representative ten publications (Past 4 years)

1) A. Higuchi*, Q.-D. Ling, S. S. Kumar, Y. Chang, T.-C. Kao, M. A. Munusamy, A. A. Alarfaj, S.-T. Hsu, A. Umezawa, External stimulus-responsive biomaterials designed for the culture and differentiation of ES, iPS, and adult stem cells, Prog Polym. Sci., 39 (2014) in press, <SCI, Impact factor= 26.383>.

2) D.-C. Chen, L.-Y. Chen, Q.-D. Ling, M.-H. Wu, C.-T. Wang, S. S. Kumar, Y. Chang, M. A. Munusamy, A. A. Alarfaj, H.-C. Wang, S.-T. Hsu, A. Higuchi*, Purification of human adipose-derived stem cells from fat tissues using PLGA/silk screen hybrid membranes, Biomaterials, 35 (2014) 4278-4287 <SCI, Impact factor= 7.604>

3) A. Higuchi*, Q.-D. Ling, S. S. Kumar, M. A. Munusamy, A. A. Alarfajj, A. Umezawa, G.-J. Wu, Design of polymeric materials for culturing human pluripotent stem cells: Progress toward feeder-free and xeno-free culturing, Prog Polym. Sci., 39 (2014) in press, <SCI, Impact factor= 26.383>.

4) S. S. Kumar, J.-S. Hsiao Q.-D. Ling, I. Dulinska-Molak, G. Chen, Y. Chang, Y. Chang, Y. H. Chen, D.-C. Chen, S.-T. Hsu, A. Higuchi*, The combined influence of substrate elasticity and surface grafted molecules on the ex vivo expansion of hematopoietic stem and progenitor cells, Biomaterials, 34 (2013) 7632-7644. <SCI, Impact factor= 7.604>

5) A. Higuchi*, Q.-D. Ling, Y. Chang, S.-T. Hsu, A. Umezawa, Physical cues of biomaterials guide stem cell differentiation fate, Chemical Reviews, 113 (2013) 3297-3328. <SCI, Impact factor= 41.298, World top No. 4 journal>

6) C.-H. Wu, Fa-Kung Lee, S. S. Kumar, Q.-D. Ling, Y. Chang, Y. Chang, H.-C. Wang, H. Chen, D.-C. Chen, S.-T. Hsu, A. Higuchi*, The Isolation and Differentiation of Human Adipose-Derived Stem Cells Using Membrane Filtration, Biomaterials, 33 (2012) 8228-8239. <SCI, Impact factor= 7.604>

7) A. Higuchi*, Q.-D. Ling, S.-T. Hsu, A. Umezawa, Biomimetic Cell Culture Proteins as Extracellular Matrices for Stem Cell Differentiation, Chemical Reviews, 112 (2012) 4507-4540. <SCI, Impact factor= 41.298, World top No. 4 journal>

8) L.-Y. Chen, Y. Chang, J.-S. Shiao, Q.-D. Ling, Y. Chang, Y. H. Chen, D.-C. Chen, S.-T. Hsu, H. H. Lee, A. Higuchi*, Effect of the surface density of nanosegments immobilized on culture dishes on ex vivo expansion  of hematopoietic stem and progenitor cells from umbilical cord blood, Acta Biomaterialia, 8 (2012) 1749-1758.<SCI, Impact factor= 5.093>

9) A. Higuchi*, P.-Y. Shen, J.-K. Zhao, C.-W. Chen, Q.-D. Ling, H. Chen, H.-C. Wang, J.-T. Bing, S.-T. Hsu, Osteoblast Differentiation of Amniotic Fluid-Derived Stem Cells Irradiated with Visible Light, Tissue Engineering, Part A, 17(21-22) (2011) 2593-2602 <SCI, Impact factor= 4.022>

10) A. Higuchi*, Q.-D. Ling, Y.-A. Ko, Y. Chang, A. Umezawa, Biomaterials for the feeder-free culture of human embryonic stem cells and induced pluripotent stem cells, Chemical Reviews, 111 (2011) 3021-3034 <SCI, Impact factor= 41.298, World top No. 4 journal>

 研究計畫

實驗室介紹

本實驗室致力於發展由奈米親水片段(奈米刷)所組成的生物醫學材料；其中臍帶血中的血液幹細胞(hematopoietic stem cells)以及組織和血液中的間質幹細胞(mesenchymal stem cells)可以被培養及保存在此生醫材料上。

我們也同時發展利用高分子薄膜來分離以及純化周邊血液和臍帶血中血液幹細胞的過濾方法。薄膜過濾法可增加血液幹細胞的數量，若應用於治療醫學上可使臍帶血中純化出的幹細胞數量增加，如此可拯救許多急需使用血液幹細胞的病患，例如白血症病患以及惡性貧血病患。

我們更進ㄧ步發展DNA生物觸媒應用於偵測血液中癌症抗原以及特殊蛋白質的生物檢驗。同時也研究利用可見光控制神經細胞突觸的生長，期盼可提供發展神經元電腦之可能性。

專利:

1.生物反應器及方法用以進行血液幹細胞之純化、體外增殖以及血液幹細胞之收集(System and method to
isolate and to proceed ex vivo culture of hematopoietic stem cells)

2.在已知化學成分之媒介中培養幹細胞利用交聯聚乙烯醇調整其表面彈性(Crosslinked polyvinylalcohol
surfaces having adjustable elasticity for stem cell culture in hemically defined media)

 專利