During the process of gene delivery, the movement of DNA vectors in 3D space is random. Only DNA vectors that diffuse to the bottom layer have the opportunity to contact cells and complete the gene transfection task. The remaining DNA molecules gradually degrade over time. This research utilizes the primary acoustic radiation force and microstreaming drag force within an Ultrasound Standing Wave Field (USWF) to enable suspended receptor cells and DNA vectors to have mutual contact opportunities every 1/2 wavelength in space, effectively increasing gene transfection efficiency.

Experiments have proven that the ultrasound standing wave field provides a more efficient method that is gentle on receptor cells and actively increases the binding of cells and DNA vectors. Furthermore, the application of the developed USWF device for gene transfection is very simple, requiring only the placement of the acoustic field chamber containing the cells on the surface of a planar acoustic transducer for a certain period. Our laboratory team is currently continuously improving the ultrasound standing wave field technology for applications in biomedical engineering, including drug delivery, tissue engineering, and bioreactors.

Due to the lack of clear early symptoms and reliable screening biomarkers for many cancers, such as ovarian cancer, cancer has become one of the deadliest diseases. Therefore, developing a highly sensitive and effective method capable of detecting early-stage malignancies is one of the most ideal goals in treatment. Detecting mRNA biomarkers of diseases in saliva is a completely non-invasive, simple, safe, and economical testing method. Currently, this technology is still in the research and development stage for cancer detection. In this study, we utilize clinical case-control study methods to attempt to find and validate salivary RNA markers for cancer and evaluate the clinical utility of this non-invasive detection method.

Improving the efficiency of gas exchange within biological culture systems has always been one of the key technologies in the research and development of cell or tissue culture techniques. Fluorochemicals are compounds with a high capacity for gas adsorption and are widely used in the biomedical industry. Our laboratory utilizes the high oxygen and carbon dioxide absorption characteristics of fluorochemicals to construct a photobioreactor system with high gas exchange efficiency. We use the marine microalgae Nannochloropsis oculata as the target cell for experiments to practically verify the efficacy of the system. Research results show that this bioreactor can effectively remove the oxygen released during the growth of Nannochloropsis oculata cells, eliminating the oxygen inhibition effect encountered during microalgae growth.

Investigating the effects of chemical or biological molecules on cardiovascular endothelial cells using static culture experimental methods has been widely studied in the past. However, under this method, endothelial cells lack the shear stress stimulation caused by actual blood flow, often leading to discrepancies between research results and clinical phenomena. To make experimental operations closer to the actual physiological environment, our research team designed a parallel-plate flow chamber system. Using this system, we study the effects of other external factors on Human Umbilical Vein Endothelial Cells (HUVECs) while simultaneously subjecting them to shear stress stimulation. Currently, the main direction of this research is to use this parallel-plate flow chamber system to extensively study the effects of nicotine on endothelial cells, such as apoptosis, aging, inflammation, and their related mechanisms.